Figure 1.

LIF-Responsive Colonies Are Derived from the Adult Periventricular Region

(A) Spherical, free-floating colonies (50–150 μm in diameter) were observed in LIF and compared with neurospheres grown in EFH (>100 μm diameter).

(B) LIF-responsive colonies gave rise to neurons (BIII tubulin⁺, green), astrocytes (GFAP⁺, purple), and oligodendrocytes (O4⁺, red).

(C) Differentiation profile of LIF colonies reveals that oligodendrocytes, neurons, and astrocytes are produced at equal frequency upon differentiation (similar to what is seen in ESC-derived pNSC colonies (C’) (n ≥ 3 colonies per group, >400 cells per colony were counted).

(D) Free-floating LIF colonies could be isolated at all time points throughout development and into old age (n > 4 per time point).

Data are shown as mean ± SEM. Scale bars represent 40 μm (A) and 50 μm (B). See also Figure S1.