Table 1.
Clonal Fibroblast Lines and the iPSC Clonal Lines Derived from Them
| Parental Cells | Clonal Fibroblasts | SNP Gene | SNP (A/B) and Location | Secondary iPSC with A Allele | Secondary iPSC with A/B Allele | Secondary iPSC with B Alleles |
|---|---|---|---|---|---|---|
| D551-iPSCK1 | dfD551-K1 | GRPR | TT/AA at 2,411–2,412 | 2 | 6 | 2 |
| 6TG-iPSC3 | df6TG-3 | GYG2 | C/T at 998 | 2 | 2 | 2 |
| GYG2 | A/G at 1,127 | |||||
| HAT-iPSC1 | dfHAT-1 | GYG2 | C/T at 998 | 2 | 3 | 1 |
| GYG2 | A/G at 1,127 | |||||
| MAOA | A/G at 4,100 | |||||
| RTT3-iPSC-46m | dfRTT3-46m | MECP2 | Del G at 705 | 4 | 3 | 2 |
| RTT4-iPSC-24w | dfRTT4-24w | MECP2 | C/T at 916 | 4 | 2 | 5 |
| RTT5-iPSC-34m | dfRTT5-34m | MECP2 | A/G at 1,461 | 2 | 4 | 3 |
| fLNS HPRT+/− | fLNS-6TG | GYG2 | C/T at 998 | 4 | 3 | 2 |
| GYG2 | A/G at 1,127 | |||||
| MAOA | A/G at 4,100 |
Fibroblast-like cells were generated from monoallelic iPSCs by differentiation. fLNS-6TG fibroblasts resistant to 6TG or fLNS-HAT fibroblasts resistant to HAT were selected from LNS fibroblasts with 6TG treatment or HAT medium. Differentiated fibroblasts, fLNS-6TG, and fLNS-HAT have monoallelic expression of genes on the X chromosome. When induced for reprogramming, iPSC clones with the same allelic expression of X-linked genes of the parental fibroblasts, iPSC clones with the opposite allele, or both are produced.