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Schematic representation of non-standard amino acid incorporation using an orthogonal translation system. Orthogonal aminoacyl tRNA synthetase, o-aaRS; orthogonal tRNA, o-tRNA; wild-type aminoacyl tRNA synthetase, wt-aaRS; wild-type tRNA, wt-tRNA; elongation factor Tu, EF-Tu,; non-standard amino acid, NSAA. Anti-codon sequence on wt-tRNA is NNN, where N is A, C, G, or U. Anti-amber codon sequence on o-tRNA is CUA.
