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Osterix expression, marking early osteoblasts, in 6-dpf mutant embryos is comparable to siblings, but expression of osteocalcin (marking mature osteoblasts) is delayed in mutant embryos. A) Osterix:nuclear-GFP+ early osteoblasts in 6-dpf sibling vs. mutant embryos. B) Osteocalcin:GFP+ mature osteoblasts are present in 6-dpf siblings, but absent in mutant embryos. C) Osteocalcin:GFP expression is observed in 12-dpf sibling vs. mutant embryos. Insets: magnification of the operculum.
