Figure 2. Distinct populations of cells, platelets and microparticles can be differentiated within whole blood, plasma and isolated cells.

(a) Platelet-poor and platelet-rich plasma, whole blood and isolated TNF-α-stimulated polymorphonuclear (PMN) cell samples were labeled with BODIPY-Maleimide dye prior to acquisition on the IS^X. Pink gates denote speed beads (and their aggregates), used as internal calibration for the cytometer; orange gate denotes erythrocytes; blue gate denotes leukocytes (neutrophils); green gate denotes platelets and aqua gate denotes microparticles, that appear as low scatter, low fluorescence intensity. (b) Representative images from each of the cell population gates.