Figure 3.
C5a did not affect HSC activation or expression of C5aR and C5L2. Mid-quiescent cells (day 5 in culture) were treated or not with C5a (10 ng/mL). Expression of (A) α-SMA, (B) Collagen1A1 and (C) C5aR mRNA was measured by qRT-PCR and normalized to 18S. Values represent means ± SEM, N = 4. Values with different superscripts are significantly different from each other (P <0.05).