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. 2014 May 24;11:95. doi: 10.1186/1742-2094-11-95

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Copyright © 2014 Neher et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Selected pro- (Fas, FasL, and Bax) and anti-apoptotic (Bcl-2) mediator levels in brain homogenates. Samples were analyzed by western blots of injured (left) and non-injured (right) hemispheres at seven days after traumatic brain injury (TBI) or sham operation. Equal concentrations of protein (50 μg per lane) were loaded on SDS-Page, and consistent loading was confirmed by β-Actin control blotting. Mouse-specific primary antibodies against Fas, FasL, Bax, and Bcl-2 were used on nitrocellulose membranes and visualized by a colorimetric assay using alkaline-phosphatase. No differences in mediator levels were detected between the groups at any time-point assessed.