Figure 2.
Inhibition of GCPII-catalyzed [3H]-NAAG hydrolysis. [3H]-NAAG was incubated with GCPII in the presence and absence of various concentrations of both monomeric and oligomeric Aβ1–40 and Aβ 1–42, as well as the GCPII prototype inhibitors 2-PMPA and 2-MPPA (Materials and Methods). Amount of [3H] glutamate produced was measured and compared to that in the absence of potential inhibitors to obtain percent inhibition. A) Monomer of Aβ1–40. B) Monomer of Aβ1–42. C) Oligomer of Aβ1–40. D) Oligomer of Aβ1–42. E) 2-PMPA IC50 = 400 pM. F) MPPA IC50 = 60 nM.