FIG. 1.

Muropeptide composition of Bacillus anthracis RCT10 (ΔcapB) mutant strain. The PG was digested with mutanolysin, reduced with sodium borohydride and separated by reverse-phase high-performance liquid chromatography using a hypersyl ODS18 column (A). The muropeptides were collected, desalted, and analyzed by MALDI-TOF and MALDI-MS/MS (Table 1). The assignment of the different peaks structures are presented in (B). G and M stand for GlcNAc (or glucosamine) and MurNAc, respectively. Ala, Gly A2pm stand for alanine, glutamate, and mesoDAP, respectively. Note that 1 volt corresponds to 1 absorbance unit at 206 nm.