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Visualization of the PDGA capsule by India ink staining. B. anthracis bacilli grown in the presence of bicarbonate were observed in the presence of India ink at the same magnification. RPG1 (A), RΔba2944 (B), and RΔba1977 (C) harbored a capsule, whereas RΔba1961 (D) and RΔba3679 (E) strains display heterogeneous and discontinuous capsule.
