FIG. 1.

Schematic outline of the GFP-PBP2x_{A707D G749V} fusion protein. GFP, green fluorescent protein GFP⁺; M, transmembrane helix; N, N-terminal domain; TP, transpeptidase/penicillin-binding domain; P1 and P2, C-terminal PASTA domains. Structural features of the P2 domain are shown in detail below: α: alpha-helix and β: beta-sheets are underlined according to³⁷; first and last aa of P2 are indicated.⁷ The mutations in GFP-PBP2x_{A707D G749V} (DKL04) are marked in red. aa, amino acids; PASTA, Penicillin-Binding Protein And Serine-Threonine-kinase Associated; PBP, and Ser/Thr-associated; PBP2x, penicillin-binding protein 2x.