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. 2014 Jun 1;20(3):250–257. doi: 10.1089/mdr.2014.0082

FIG. 3.

FIG. 3.

Cellular localization of the GFP-fusion protein in DKL041. (A) Top: PBP profile analysis. PBPs in cell lysates were labeled with Bocillin™ FL and visualized by fluorography. Bottom: Western blot developed with anti-PBP2x antibodies. The positions of PBPs and GFP-2x* (GFP-PBP2xA707D G749V in strain DKL04 and GFP-PBP2xG749V in strain DKL041) are indicated on the right side. + and − indicate growth with and without ZnCl2. Strains used for preparation of cell lysates are indicated on top. (B) Localization of GFP-PBP2xG749V in DKL041 strain. Top: Phase contrast microscopy; Bottom: Fluorescence signal. Scale bar=2 μm.