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. 2014 Jun 1;20(3):250–257. doi: 10.1089/mdr.2014.0082

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 4. — Effect of the mutation D707. (A) Top. PBP in cell lysates of strains DKL03 (R6, P_Zn-gfp-pbp2x) and DKL22 (R6, P_Zn-gfp-pbp2x_A707D) were labeled with Bocillin FL and visualized by fluorography. Bottom: Western blot of the gel on the top developed with anti-PBP2x antibodies. + and − indicate growth with and without ZnCl₂. The positions of the PBPs and GFP-2x* (GFP-PBP2x and GFP-PBP2x_A707D) are indicated on the right. Strains used for preparation of cell lysates are indicated on top. (B) Micrographs of strain DKL22 grown in the presence of ZnCl_2. Top: Phase contrast microscopy; Bottom: Fluorescence signal. Scale bar=2 μm.