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. 2014 Apr 18;2(5):576–591. doi: 10.1016/j.stemcr.2014.03.003

Figure 2.

Figure 2

Adult Human CS-Derived Cells Differentiate into Immature Cardiomyocytes

(A) Fetal and adult HDCs formed CSs after 1 week in suspension culture (a and i). CS-derived cells formed microtubules after 24–48 hr in Matrigel angiogenesis assay (b and j). CS-derived cells were plated on coverslips treated with laminin for 15 days to induce cardiovascular differentiation. Confocal images showed expression of vWF (c and k), CNN (d and l), and cardiac proteins cTNT (green) and cTNI (red) (e–h and m–p). Sarcomeres were rare in adult CS-derived CM-like cells (m–p). Nuclei were stained with DAPI (blue). Scale bars, 40 μm.

(B) Cardiovascular differentiation (Diff) of HDCs and CS-derived cells pre- and postdifferentiation was quantitated by FACS. All experiments were performed with a minimum of three independent human heart samples. ∗p < 0.05.