Figure 6.

Sirt1^−/− iPSCs Show Higher Chromosomal Instability

(A–C) Quantification of multitelomeric signals (MTS) (A), chromosome breaks (B), and chromosome fusions (C) in Sirt1^+/+ and Sirt1^−/− MEFs (passage 3; two independent cell lines per genotype) and iPSCs (passage 5; two independent clones per each parental MEF). A representative image of every chromosomal aberration is shown. Error bars represent the SEM. Statistical analysis was performed using a two-tailed Student’s t test.

(D and E) SKY analysis of metaphase spreads from Sirt1^+/+ and Sirt1^−/− iPSCs (passage 35). Each chromosome is represented from left to right by DAPI staining, the display color, and the classification pseudocolor. Karyotype formulas: 39−42.XY,+2,+8 (D); 35–41.X0,rob(6;6),+8,der(8)T(8;X),+17×2 (E).

See also Figure S4.