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. 2014 Apr 17;2(5):592–605. doi: 10.1016/j.stemcr.2014.03.006

Figure 1.

Figure 1

Functionality of the TBX3 Overexpression Construct in ESCs

(A) Twenty independent cell clones were stably transfected with an overexpression construct bearing the human TBX3-cDNA. Overexpression levels were analyzed via qRT-PCR. Four representative clones were selected for further analysis. The data represent two biological replicates and are presented as means ± SD.

(B) Immunostaining of overexpressed TBX3 (red), Actin (green), and nuclei (blue) in the four selected clones confirm overexpression levels of TBX3. Scale bar, 10 μm.

(C) FACS analyses of OCT-4/POU5f1 and SOX2 reveal no influence of TBX3 overexpression on pluripotency under LIF administration. The data represent five independent experiments and are presented as means ± SEM.