(A) Flow cytometry of lung-infiltrating leukocyte populations in C57BL/6 mice at indicated time-points after poly I:C administration, with the gating indicated above the plots. Upper-right panel, percentage of NK1.1+, TCR-β+, or TCR-γδ+ cells within total live cells from the lung; lower-right panel, percentage of CD4+ or CD8+ cells within total live cells from the lung. SSC, Side-scatter. (B) C57BL/6 mice were pretreated with anti-NK1.1, 2 days prior to poly I:C injection. IFN-γ and chemokine mRNA levels in the lung were measured 6 h after poly I:C injection. All data are from analyses of five individual mice in each group (two mice/experiment; total of two independent experiments).