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. 2012 Nov;92(5):999–1009. doi: 10.1189/jlb.0512242

Figure 1. i.v. administration of α-GalCer induces IL-4 reporter expression in NKT cell in G4 mice.

Figure 1.

(A) Representative dot plots showing expression of the IL-4 reporter on gated NKT cells (CD1d Tetr-binding cells, as shown in Supplemental Fig. 1) from spleen and liver of heterozygous G4 mice on a BALB/c background administered 200 ng α-GalCer, relative to nontransgenic and untreated control animals. (B) Mean percentages of IL-4 reporter-positive cells (±sem) within the CD4+ and CD4− NKT cell subsets of the indicated tissues over time after α-GalCer administration (n=4–6 animals/group). Significant differences between the subsets were determined by two-way ANOVA with Bonferroni post-test. Tissues were also examined from similarly treated homozygous G4 mice and mice homozygous for the G4 reporter and STAT6 deficiency. A graph showing levels of IL-4 protein in the serum is also presented. (C) Representative dot plots from a similar analysis in G4 mice on a C57BL/6 background.