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. 2014 Jun 10;4:73. doi: 10.3389/fcimb.2014.00073

Figure 1.

Figure 1

(A) A schematic diagram of the envelope structure of C. trachomatis. Showing are the representative OM associated components [Lipopolysaccharide (LPS), peptidoglycan (PG), MOMP, Pmps, OmcA, OmcB, and Omp85], and putative chaperone and protease in the periplasm in EBs. The potential process of MOMP or other OM protein transport across the inner membrane via the Sec pathway, and insertion and assembly into the OM is indicated. (B) Immunoblot of C. trachomatis serovar F Omp85. Similar to MOMP, Omp85 is present in both EBs and RBs as a component of the OM complex (OMC), however, OmcB exists only in EBs. The OMCs were prepared from the insoluble fraction of isolated EBs or RBs in phosphate buffered saline containing 2% Sarkysol as described previously (Caldwell et al., 1981). The lysates of EBs and RBs, and their OMCs were dissolved in Laemmli sample buffer (1:1, v:v) supplemented with 5% 2-mercaptoethanol, 10 mM dithiothreitol and heated for 10 min at 100°C, isolated on 10% SDS-PAGE, followed by immunoblotting using polyclonal antibodies to Omp85 or OmcB, and a monoclonal antibody to C. trachomatis serovar F MOMP.