Figure 3.

Micrograph of chlamydial MVs in cervical epithelial cells from C. trachomatis infected patients. (A,B) Presence of C. trachomatis vesicles associated with an aberrant C. trachomatis form in an inclusion. Vesicles appeared in the form of a chain or single round vesicular structures. This sample was obtained from a patient (patient 2) presented in Lewis et al. in this journal (Lewis et al., 2014); (C,D) presence of C. trachomatis MVs associated with a normal C. trachomatis form in an inclusion obtained from an epithelial cell from a second C. trachomatis infected patient. C. trachomatis infected human epithelial cells were collected by cytobrush from patients with an NAAT and culture positive C. trachomatis infection, and were immediately placed in a modified TEM fixative (Lewis et al., 2014) (currently under review for this Journal) and processed as previously described (Belland et al., 2003a). Scale bar = 500 nm.