Table 2.

General properties of recombinant 3-hydroxypropionyl-CoA synthetase and recombinant 4-hydroxybutyryl-CoA synthetase from N. maritimus

Property	3-Hydroxypropionyl-CoA synthetase	4-Hydroxybutyryl-CoA synthetase
Reaction catalyzed	3-hydroxypropionate + ATP + CoA → 3-hydroxypropionyl-CoA + ADP + Pi	4-hydroxybutyrate + ATP + CoA → 4-hydroxybutyryl-CoA + ADP + Pi
Gene	Nmar_1309	Nmar_0206
Molecular mass, kDa	76	76
Predicted molecular mass, kDa*	76.1	75.6
Divalent cations required for the activity	Mg2+ or Mn2+ (no activity with Ni2+, Co2+, and Ca2+)	Mg2+ or Mn2+ (no activity with Ni2+, Co2+, and Ca2+)

^*Predicted for the native protein without polyhistidine tags.