Fig. 4.

Up- and down-regulated pathways and clades in the gut metatranscriptome. Gene and transcript relative abundances are generally well correlated (Spearman’s r = 0.76). (A–H) Each scatterplot illustrates the average gene (DNA) and transcript (RNA) relative abundance for 3,292 KOs from the eight frozen HPFS stool samples, highlighting a prominent over- or underexpressed functional module. Red circles correspond to KOs where RNA > DNA; blue circles correspond to KOs where DNA > RNA. Marks on the x or y axis margins represent KOs with zero measured abundance in one dataset but nonzero abundance in the other. The trends illustrated here were all of large effect (fold-change > 2) and statistically significant following FDR correction (Methods).