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. 2014 Jun 10;4:77. doi: 10.3389/fcimb.2014.00077

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Copyright © 2014 Heimesaat, Alutis, Grundmann, Fischer, Tegtmeyer, Böhm, Kühl, Göbel, Backert and Bereswill.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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C. jejuni colonization along the intestinal tract of gnotobiotic IL-10^−/− mice is independent of htrA gene expression. (A) Control Western blots for the expression of indicated proteins in the two C. jejuni strains used in this infection study. (B) Gnotobiotic IL-10^−/− mice were generated by broad-spectrum antibiotic treatment and perorally infected with C. jejuni NCTC11168 wild-type strain (WT, closed circles) or mutant strain NCTC11168ΔhtrA (ΔhtrA, open circles). The pathogen densities in distinct compartments of the intestinal tract were determined by quantification of live C. jejuni in luminal samples taken from duodenum, ileum, and colon at day 6 p.i. by cultural analysis (CFU, colony forming units). Medians (black bars) are indicated and numbers of analyzed animals given in parentheses. Data shown were combined from five independent experiments.