Figure 6.

Influence of BIBF1120 on pairing stability and egg production on S. mansoni couples in vitro. Treatment of S. mansoni couples with BIBF1120 for 96 h in vitro revealed a concentration-dependent effect of the FGFR inhibitor on pairing stability (A) and egg production (B) compared to the control (DMSO). Whereas 1 μM BIBF1120 had no influence on pairing, couples treated with 5 μM separated during the experiment period. The highest inhibitor concentration (10 μM) supplied, led to a separation of all couples within the first 24 h (A). An obvious effect of BIBF1120 on egg production was also detected after 24 h. At this time point the inhibitor treatment led to a decrease of eggs down to 50% (1 μM) and to 20% (5 μM) compared to the control. At higher concentrations egg production was nearly completely disrupted (B). In both diagrams the statistical evaluation of three independent experiments is shown (error bars indicated).