Fig. 2.

Schematic of the algorithm for identifying whole organ volume differences. Images from eight wild-type and eight knockout mouse embryos are registered into a population average image. The 3D segmented atlas (Wong et al., 2012) is then registered to this population average such that each segmented structure is resampled onto the population average space to provide a segmented population atlas. The segmented structures can then be back propagated onto the native images using the inverse of the transformations, which allows for automated volume measurements of the 48 structures.