FIG. 2.

Quantification of goblet cells using Alcian blue and periodic acid Schiff reagent (AB–PAS) staining. (A) Representative pictures of maximum and minimum numbers of goblet cells in the intestinal bulb, the mid-intestine, and the posterior intestine. Histochemical staining with AB–PAS demonstrates that goblet cells (blue) synthesize acidic mucins. (B) Goblet cell numbers. All error bars are represented as mean±SEM. n=10 larvae per group, *p<0.05, **p<0.01, and ***p<0.001. Color images available online at www.liebertpub.com/zeb