FIG. 3.

Immunofluorescence analysis of tumor necrosis factor (TNF)-α expression in the gut. (A–F) Representative cross-sections of the mid-intestine from different groups. (G) Densitometric analysis of multiple similar experiments. TNF-α staining (red) and DAPI staining (blue) images were visualized by confocal laser scanning microscopy. Cross-sections of the mid-intestine from at least six fish were examined for each condition. Images were shown at 200× magnification. Error bars are represented as mean±SEM, n=13–16 sections per group. P values were calculated using a paired t-text, ***p<0.001. Color images available online at www.liebertpub.com/zeb