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TNBS exposure increased the expression of TLR3, TRIF, and MyD88 in CV conditions and XGF zebrafish. No detectable expression of TLR3 and no obvious stimulation of MyD88 or TRIF in GF fish. (A) Western blotting with anti-TLR3, anti-TRIF, or anti-MyD88 antibodies. (B) Densitometric analysis of multiple similar experiments. Data are means±SEM; n=4 or 5. P values were calculated using a paired t-test, **p<0.01, and ***p<0.001.
