FIG. 2.

Amino acid sequence alignment of yellow catfish (T. fulvidraco) Mstn with other representative vertebrates' Mstn. The amino acid identities of Mstns from different animals were determined using MEGA5. Identical amino acid residues are cross hatched and dashes represent gaps for alignment purposes. The predicted proteolytic site (RX) to remove the signal sequence, the proteolytic site (RSSR) to generate bioactive form of Mstn, and the conserved nine cysteine residues in the bioactive domain are boxed. zf: zebrafish; X: Xenopus tropicalis; yc: yellow catfish; ch: chicken; h: human.