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. Author manuscript; available in PMC: 2014 Jun 10.
Published in final edited form as: J Photochem Photobiol A Chem. 2012 Aug 15;248:24–29. doi: 10.1016/j.jphotochem.2012.07.012

Table 1.

FRET parameters obtained from time-resolved fluorescence measurements.

Sample RITC:NCa A1b τD(1) / ns A2b τD(2) / ns r / Åc E/% kFRET / s−1
CdSe/ZnS NC 0.45 16.9 0.55 2.01
RITC-NC 2.5:1 0.56 6.1 0.44 0.87 38 64 1.2 × 108
RITC-NC 0.8:1 0.56 6.2 0.44 0.53 32 63 3.5 × 108
RITC-NC 0.3:1 0.49 9.2 0.51 0.12 30 45 3.0 × 108
a

dye-to-NC ratio.

b

pre-exponetial weighting factor of biexpoential fit.

c

corrected for Poisson distribution statistics as discussed in ref. 52, r = 38.8 nm for the 2.5:1, r = 31.4 for 0.8, and r = 32.1 for the 0.3:1 dye/NC samples.