Table 1.
Sample | RITC:NCa | A1b | τD(1) / ns | A2b | τD(2) / ns | r / Åc | E/% | kFRET / s−1 |
---|---|---|---|---|---|---|---|---|
CdSe/ZnS NC | – | 0.45 | 16.9 | 0.55 | 2.01 | – | – | – |
RITC-NC | 2.5:1 | 0.56 | 6.1 | 0.44 | 0.87 | 38 | 64 | 1.2 × 108 |
RITC-NC | 0.8:1 | 0.56 | 6.2 | 0.44 | 0.53 | 32 | 63 | 3.5 × 108 |
RITC-NC | 0.3:1 | 0.49 | 9.2 | 0.51 | 0.12 | 30 | 45 | 3.0 × 108 |
dye-to-NC ratio.
pre-exponetial weighting factor of biexpoential fit.
corrected for Poisson distribution statistics as discussed in ref. 52, r = 38.8 nm for the 2.5:1, r = 31.4 for 0.8, and r = 32.1 for the 0.3:1 dye/NC samples.