Table 2.

SAXS-derived parameters

DOI: http://dx.doi.org/10.7554/eLife.02674.007

Buffer conditions	c (mg/ml)	MW (kDa)	Rg (nm)	Dmax (nm)	χexp
20 mM Tris pH 7.5 300 mM NaCl 1 M NDSB-201	2.00	93	4.09	13.71	0.983
–	1.50	89	3.90	13.22	0.908
–	1.00	82	3.84	13.01	0.857
20 mM Tris pH 7.5 150 mM NaCl 500 mM NDSB-201	0.75	87	3.52	12.60	0.839
20 mM Tris pH 7.5 150 mM NaCl 500 mM NDSB-201 + 1 M Gdn-HCl	0.50	124	4.24	14.50	0.829
20 mM Tris pH 7.5 150 mM NaCl 500 mM NDSB-201 + 2 M Gdn-HCl	0.50	140	4.72	15.34	0.856
20 mM Tris pH 7.5 150 mM NaCl 500 mM NDSB-201 + 3 M Gdn-HCl	0.50	88	5.24	18.35	0.893
20 mM Tris pH 7.5 1.15 M NaCl 250 mM AMP	1.80	85	4.30	14.70	0.986
20 mM Tris pH 7.5 150 mM NaCl 250 mM AMP	2.00	97/78*	4.01	13.26	>10
20 mM Tris pH 7.5 150 mM NaCl 500 mM NDSB-201 250 mM AMP	0.75	90	3.91	13.70	0.814
20 mM Tris pH 7.5 150 mM NaCl 500 mM NDSB-201 250 mM UMP	0.75	92	3.86	13.50	0.843
20 mM Tris pH 7.5 150 mM NaCl 500 mM NDSB-201 250 mM CMP	0.75	87	3.99	13.98	0.808
20 mM Tris pH 7.5 150 mM NaCl 500 mM NDSB-201 + 5 mM EDTA	0.75	82	3.86	14.2	0.927
20 mM Tris pH 7.5 575 mM NaCl	0.4	234	4.83	17.2	N.D
20 mM Tris pH 7.5 3 M NaCl	0.4	463	8.62	26.7	N.D
20 mM Tris pH 7.5 1.15 M NaCl	0.4	178	5.77	20.10	N.D
20 mM Tris pH 7.5 1.15 M NaCl	0.90	254	5.91	20.50	N.D
20 mM Tris pH 7.5 1.15 M NaCl	1.50	305	6.10	21.30	N.D
20 mM Tris pH 7.5 1.15 M NaCl	3.00	580	7.20	25.20	N.D

^*97 kDa assuming 90% protein + 10% RNA and 78 kDa assuming only protein.