Figure 2.

Multi-epitope peptide-specific cytokine production by CD4⁺ and CD8⁺ T cells. Spleen cells from BALB/c mice (n = 5/group) immunized either with individual peptide emulsified in CFA/IFA or PBS alone, were re-stimulated in vitro with the respective peptide (10 μg/ml) for 48 h, and analyzed for CD4⁺ and CD8⁺ T cells producing IL-4 and IFN-γ. (A–C) Representative FACS plots of intracellular staining used to define IL-4- and IFN-γ-expressing CD4⁺ and CD8⁺ T cells in spleens derived from immunized mice. Values represent the percentages of (A) IL-4⁺ cells among CD4⁺ T cells and (B,C) IFN-γ⁺ cells among CD4⁺ and CD8⁺ T cell populations. (D) Quantification of IL-4 and IFN-γ producing T cells in immunized mice. All data were normalized to CFA/IFA control group. Each bar represents the mean proportion of IL-4- and IFN-γ-producing CD4⁺ and CD8⁺ T cells induced by the respective peptide.