Table 3.
Protein | No. of epitope | Epitope sequence | Scorea |
||
---|---|---|---|---|---|
SYFPEITHI |
NetMHCII |
||||
H2-IAd | H2-IEd | H2-IAd | |||
CPA | 1 | 149-MCGSCWAFATTGNIE-163 | 28 | – | – |
2 | 246-PHDEEEIAAYVGKNG-260 | 27 | – | – | |
3 | 114-KDYKEHVHVDDSVRS-128 | 26 | – | – | |
4 | 257-GKNGPVAVAVDATTW-271 | 26 | – | – | |
5 | 32-GVDDFIASAHYGRFK-46 | 25 | – | – | |
6 | 312-GSSWGEKGYIRLAMG-326 | – | 24 | – | |
7 | 4-RNPFFFAIVVTILFV-18 | 23 | – | – | |
8 | 5-NPFFFAIVVTILFVV-19 | 23 | – | – | |
9 | 13-VTILFVVCYGSALIA-27 | 22 | – | – | |
10 | 172-SLVSLSEQVLVSCDN-186 | 22 | – | – | |
11 | 174-VSLSEQVLVSCDNID-188 | 22 | – | – | |
12 | 12-VVTILFVVCYGSALI-26 | 21 | – | – | |
13 | 260-GPVAVAVDATTWQLY-274 | 21 | – | – | |
14 | 279-VTLCFGLSLNHGVLV-293 | 21 | – | – | |
15 | 67-KQNMQTAYFLNAHNP-81 | 20 | – | – | |
16 | 273-LYFGGVVTLCFGLSL-287 | 20 | – | – | |
17 | 301-KPPYWIVKNSWGSSW-315 | 20 | – | – | |
18 | 328-NQCLLKNYVVTATID-342 | 20 | – | – | |
19 | 216-SYPYTSAGGTRPPCH-230 | – | 20 | – | |
20 | 308-KNSWGSSWGEKGYIR-322 | – | 20 | – | |
Histone H1 | 21 | 1-MSSDSAVAALSAAMT-15 | 31 | – | 88.3 |
22 | 27-KTAAKKAAAKKAAAK-41 | 29 | – | 182.6 | |
23 | 32-KAAAKKAAAKKAGAK-46 | 29 | – | 239.9 | |
24 | 37-KAAAKKAGAKKAGAK-51 | 29 | – | – | |
25 | 42-KAGAKKAGAKKAVRK-56 | 29 | – | – | |
26 | 2-SSDSAVAALSAAMTS-16 | 28 | – | 123.7 | |
27 | 56-KVATPKKPAKKAAKK-70 | 24 | – | – | |
28 | 36-KKAAAKKAGAKKAGA-50 | – | 24 | – | |
29 | 41-KKAGAKKAGAKKAVR-55 | – | 24 | – | |
30 | 71-AAKKPAKKVAKKPAK-85 | – | 24 | – | |
31 | 16-SPQKSPRSSPKKTAA-30 | – | 22 | – | |
32 | 21-PRSSPKKTAAKKAAA-35 | – | 22 | – | |
33 | 26-KKTAAKKAAAKKAAA-40 | – | 22 | 163.5 | |
34 | 31-KKAAAKKAAAKKAGA-45 | – | 22 | 190.3 | |
35 | 45-AKKAGAKKAVRKVAT-59 | – | 22 | – | |
36 | 51-KKAVRKVATPKKPAK-65 | – | 22 | – | |
37 | 55-RKVATPKKPAKKAAK-69 | – | 22 | – | |
38 | 59-TPKKPAKKAAKKAAK-73 | – | 22 | – | |
39 | 63-PAKKAAKKAAKKPAK-77 | – | 22 | – | |
40 | 67-AAKKAAKKPAKKVAK-81 | – | 22 | – | |
41 | 75-PAKKVAKKPAKKAAK-89 | – | 22 | – | |
42 | 79-VAKKPAKKAAKKPAK-93 | – | 22 | – | |
43 | 83-PAKKAAKKPAKKPAK-97 | – | 22 | – | |
44 | 87-AAKKPAKKAAKKAAK-101 | – | 22 | – | |
45 | 91-PAKKPAKKAAKKAAK-105 | – | 22 | – | |
46 | 95-PAKKAAKKAAKKAAA-109 | – | 22 | – | |
47 | 5-SAVAALSAAMTSPQK-19 | 21 | – | 382.8 | |
48 | 76-AKKVAKKPAKKAAKK-90 | 21 | 20 | – | |
49 | 96-AKKAAKKAAKKAAAK-110 | 21 | – | – | |
50 | 29-AAKKAAAKKAAAKKA-43 | – | – | 107.9 | |
51 | 30-AKKAAAKKAAAKKAG-44 | – | – | 119.1 | |
52 | 25-PKKTAAKKAAAKKAA-39 | – | – | 119.6 | |
53 | 24-SPKKTAAKKAAAKKA-38 | – | – | 138.5 | |
54 | 3-SDSAVAALSAAMTSP-17 | – | – | 166.2 | |
55 | 28-TAAKKAAAKKAAAKK-42 | – | – | 183.3 | |
56 | 35-AKKAAAKKAGAKKAG-49 | – | – | 208.6 | |
57 | 34-AAKKAAAKKAGAKKA-48 | – | – | 232.0 | |
58 | 4-DSAVAALSAAMTSPQ-18 | – | – | 272.1 | |
59 | 48-AGAKKAVRKVATPKK-62 | – | – | 295.2 | |
60 | 33-AAAKKAAAKKAGAKK-47 | – | – | 317.8 | |
61 | 49-GAKKAVRKVATPKKP-63 | – | – | 457.6 | |
KMP-11 | 62 | 4-TYEEFSAKLDRLDEE-18 | 23 | – | – |
63 | 75-QKFAELLEQQKAAQN-89 | 19 | – | – | |
64 | 45-KEHYEKFERMIKEHT-59 | – | 18 | – | |
65 | 74-KQKFAELLEQQKAAQ-88 | – | 18 | – | |
LeIF | 66 | 100-LSPTRELALQTAEVI-114 | 28 | – | 264.2 |
67 | 320-VLVTTDLVARGICVH-334 | 28 | – | – | |
68 | 199-LPKDIQVALFSATMP-213 | 27 | – | – | |
69 | 387-HTQIDELPVDFAAYL-401 | 27 | – | – | |
70 | 138-QDDLRKLQAGVIVAV-152 | 26 | – | 205.3 | |
71 | 166-ALRTESLRVLVLDEA-180 | 26 | – | – | |
72 | 169-TESLRVLVLDEADEM-183 | 26 | – | – | |
73 | 62-RGGDIIAQAQSGTGK-76 | 25 | – | – | |
74 | 140-DLRKLQAGVIVAVGT-154 | 24 | – | 256.8 | |
75 | 142-RKLQAGVIVAVGTPG-156 | 24 | – | – | |
76 | 259-MDLYETVSIAQSVIF-273 | 24 | – | – | |
77 | 223-FMRDPVRILVKRESL-237 | – | 24 | – | |
78 | 325-DLVARGIDVHHVNIV-339 | 23 | – | – | |
79 | 168-RTESLRVLVLDEADE-182 | 22 | – | – | |
80 | 263-ETVSIAQSVIFANTR-277 | 22 | – | – | |
81 | 293-TVSSMHAEMPKSDRE-307 | 22 | – | – | |
82 | 314-RSGSSRVLVTTDLVA-328 | 22 | – | – | |
83 | 268-AQSVIFANTRRKVDW-282 | – | 22 | – | |
84 | 16-LDDQPGVRPIPSFDD-30 | 20 | – | – | |
85 | 71-QSGTGKTGAFSIGLL-85 | 20 | – | – | |
86 | 107-ALQTAEVISRIGEFL-121 | 20 | – | – | |
87 | 174-VLVLDEADEMLSQGF-188 | 20 | – | – | |
88 | 255-LDTLMDLYETVSIAQ-269 | 20 | – | – | |
89 | 376-VELLHEIEAHYHTQI-390 | 20 | – | – | |
90 | 77-TGAFSIGLLQRLDFR-91 | – | 20 | – | |
91 | 81-SIGLLQRLDFRHNLI-95 | – | 20 | – | |
92 | 158-VSDVIKRGALRTESL-172 | – | 20 | – | |
93 | 102-PTRELALQTAEVISR-116 | – | – | 95.3 | |
94 | 103-TRELALQTAEVISRI-117 | – | – | 95.4 | |
95 | 101-SPTRELALQTAEVIS-115 | – | – | 163.8 | |
96 | 139-DDLRKLQAGVIVAVG-153 | – | – | 249.0 | |
97 | 99-VLSPTRELALQTAEV-113 | – | – | 263.1 | |
98 | 98-LVLSPTRELALQTAE-112 | – | – | 287.6 | |
99 | 141-LRKLQAGVIVAVGTP-155 | – | – | 296.7 | |
100 | 49-PSSIQQRAIAPFTRG-63 | – | – | 320.9 | |
101 | 48-KPSSIQQRAIAPFTR-62 | – | – | 378.0 | |
102 | 50-SSIQQRAIAPFTRGG-64 | – | – | 434.6 | |
103 | 97-GLVLSPTRELALQTA-111 | – | – | 452.0 | |
104 | 290-SNHTVSSMHAEMPKS-304 | – | – | 489.1 |
aThe cut-off score was adjusted to ≥18 for SYFPEITHI, ≥100 for BIMAS, and <500 for NetMHCII.