Table 1.
Organs and tissues used in the RRIM600 and RRIM600 OPS RNA extracts
| RRIM600 | RIN* | RRIM600 OPS | RIN |
|---|---|---|---|
| Apical meristem |
3.0 |
Cotyledon in the seed (germination stage I) |
4.7 |
| Leaflets stage A |
3.3 |
Seedlings (germination stage II) |
5.3 |
| Leaflets stage B2 |
1.8 |
Stalk (germination stage III) |
3.7 |
| Leaflets stage C |
2.7 |
Roots (germination stage III) |
3.7 |
| Leaflets stage D |
2.5 |
Seed (germination stage III) |
3.3 |
| Petiole (Leaf stage B2) |
2.1 |
Leaves (germination stage IV) |
1.6 |
| Petiole (Leaf stage C) |
2.7 |
Stalk (germination stage IV) |
3.5 |
| Petiole (Leaflets stage D) |
2.7 |
Roots (germination stage IV) |
3.4 |
| Lignified stem |
3.0 |
Seed (germination stage IV) |
3.3 |
| Bark (trunk grafted with MDF180 crown) |
3.1 |
Immature seeds with transparent endosperm |
2.2 |
| Bark (trunk and crown of RRIM600) |
2.8 |
Immature seeds with white endosperm |
1.2 |
| Latex |
4.1 |
Fertilized female flowers |
4.5 |
| Raceme |
2.9 |
|
|
| Male flowers, mature and immature |
3.7 |
|
|
| Columns and wall of fruit lobes |
3.3 |
|
|
| Fruit peel |
3.4 |
|
|
| Peduncle |
2.8 |
|
|
| Seed stage A endosperm | 1.2 |
Leaf development: Stage A: the preformed leaves in the terminal bud appear; Stage B2: upward leaflets of small size are anthocyanic initially. Then, the lamina reverses and bends to the ground and the anthocyanic color diminishes; Stage C: Rapid lamina growth. The flabby and pale green lamina maintains a drooping position; Stage D: leaves straighten into a horizontal position and become stiff; Seed germination. Stage I: appearance of the radicle; Stage II: appearance of the primary root, side roots and stalk; Stage III: elongation of the primary root, side roots and stalk; Stage IV: elongation of the primary root, side roots and growth of the first two branches. *The RIN software algorithm allows classification of total RNA, based on a numbering system from 1 to 10, with 1 being the most degraded profile and 10 being the most intact.