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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Cancer Res. 2014 Mar 19;74(10):2857–2868. doi: 10.1158/0008-5472.CAN-13-2003

Figure 3. RUNX2 modifies AR localization and mobility in living cells.

Figure 3

A. C4-2B/Rx2dox cells were treated with dox and/or DHT, then immunostained and subjected to confocal microscopy to visualize the AR (green) and RUNX2 (red). DAPI (blue) demarcates the cell nucleus. B. GFP-AR or GFP-AR-A573D fusion proteins were expressed in COS7 cells either alone or together with RUNX2. The cells were treated with DHT and a portion of their nuclei was subjected to FRAP analysis. Curves represent fluorescence intensity relative to the respective pre-photobleaching levels.