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. 2013 Nov 26;28(6):1308–1315. doi: 10.1038/leu.2013.326

Figure 1.

Figure 1

Representative FACS profiles of CB-derived 18Lin−CD45+CD34+/−CD133+/− cells. (a) The FSC/SSC profile of immunomagnetically separated Lin− cells. The R1 gate was set on the blast–lymphocyte window. (b) The R2 gate was set on the 18Lin− living cells. (c) The 18Lin− living cells (R2) were subdivided into two fractions: 18Lin−CD45+CD34+ (R3) and CD34− (R4) cells, according to their expression levels of CD34. The definitions of CD34+/− cells are as follows: the CD34+ fraction contains cells expressing maximum APC fluorescence intensity (FI) to 5% the level of FI. The CD34− level of FI was determined based on the Fluorescence Minus One controls. (d) The 18Lin−CD34+ cells residing in the R3 gate were further subdivided into two fractions: 18Lin−CD45+CD133+ (R5) and CD133− (R6) cells, according to their expression levels of CD133. The definitions of CD133+/− cells are as follows: the CD133+ fraction contains cells expressing maximum PE FI to 15% the level of FI and the CD133− level of FI was determined based on the Fluorescence Minus One controls. (e) The R4-gated cells were further subdivided into two fractions: 18Lin−CD45+CD34−CD133+ (R7) and CD133− (R8) cells. The definitions of CD133+/− cells are the same as described above.