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Comparison of catalysts in 2-chloro-4-nitrophenyl glycoside driven sugar nucleotide syntheses. A) General reaction scheme. B) Maximum observed percentage conversion of (U/T)DP to (U/T)DP-sugars by OleD Loki (dark), TDP-16 (light dark) and wtOleD (gray) (n ≥ 2, S. D. < 8%) (See Supplement methods). C) Additional 2-chloro-4-nitophenyl glycosides tested for which no products were observed within the sensitivity limits of the assay.
