Skip to main content
. 2013 Dec;94(6):1293–1301. doi: 10.1189/jlb.1012524

Figure 5. Results of DC-adoptive transfer.

Figure 5.

Adoptive transfer of WT or TSP-1KO DCs into WT or TSP-1KO mice. Bone marrow-derived DCs from WT or TSP-1KO mice were adoptively transferred into WT and TSP-1KO mice that were allowed to rest for 72 h. Dry -eye phenotype was evaluated before (NS) and after DS5. (A) Representative dot plot of unattached, cultured bone marrow-derived cells at Day 9, double-stained for CD11c and CD11b. Numbers in the quadrants indicate the percentage of positive cells. (B) Representative immunohistochemistry of conjunctival frozen sections obtained from DC recipient mice showing staining of TSP-1-positive cells (in red, black arrows). (C) Representative images of OGD corneal staining that were used to an generate OGD intensity score in WT and TSP-1KO after DC adoptive transfer. (D) Bar graphs show mean ± sd of three independent experiments using 15 mice for each group/time-point/experiment. Note that reconstitution of TSP-1KO with WT DC impairs TSP-1KO resistance to desiccation, whereas transfer of TSP-1KO-DC does not. (E) CD4+ T cell infiltration of the conjunctival epithelium in the CG-rich area. Bar graphs show mean ± sd of two independent experiments (final n=5 for each group). (F) PAS+ conjunctival GC density. Bar graphs show mean ± sd of two independent experiments (final n=5 for each group). *P < 0.05; **P < 0.01; ***P < 0.001.