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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Protein Expr Purif. 2013 Apr 28;90(1):1–8. doi: 10.1016/j.pep.2013.04.008

Figure 1.

Figure 1

gE structural features and PCR amplification (A) GC content of the gE gene. 50 bp sliding window scan of the gE gene open reading frame. The overall GC content is 75%, ranging from 56% to a high of 94% (B) PCR amplification of the gE gene using betaine as a PCR enhancer. Lane 1: 1000 bp marker (NEB), Lane 2: PCR reaction. (C) Diagram of the recombinant gE gene product. N-linked glycosylation sites, the predicted transmembrane domain, as well as the position of tags picked up from the baculoviral vector are indicated.