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. Author manuscript; available in PMC: 2014 Jun 10.
Published in final edited form as: Methods Mol Biol. 2014;1071:29–47. doi: 10.1007/978-1-62703-622-1_3

Fig. 4.

Fig. 4

The value of the Hill coefficient (n) does not affect the sensor’s Kd’ if the data are fit using the equations in Fig. 3. n affects the change in the FRET ratio resulting from a change in the [ion]. For example, if the [ion] changes from 2 × Kd’ to 4 × Kd’, the change in FRET ratio is greater when n is larger.