Table 2.
Selected ratiometric Zn2+ biosensors
| Sensor | Zn2+- responsive elements |
Kd’ for Zn2+ |
Hill coefficient |
Comments | References | |
|---|---|---|---|---|---|---|
| eCALWY family |
eCALWY4 | Atox1 and the WD4 domain of ATP7B |
630 pM | 1 | Optimized for cytosol |
(3) |
| ZinCh family |
eZinCh | Zn2+- coordinating residues on CFP and YFP connected by a flexible linker |
8.2 μM | 1 | Targeted to vesicles by fusion to VAMP2 |
(3, 21) |
| Zap family | ZapCY1 | Zap | 2.53 pM |
1 | Optimized for ER, Golgi, and mitochondria; high dynamic range |
(5) |
| Zap family | ZapCY2 | mZap | 811 pM | 0.44 | Optimized for cytosol |
(5) |