Table 4.
Chemicals used for Ca2+ and Zn2+ calibrations
| Chemical | Working concentration |
Effect | Comments and References |
|---|---|---|---|
| Ionomycin Bromo-A23187 |
5-10 μM 0.1 – 20 μM |
Ca2+ ionophore | |
| TPEN | 50-150 μM | Zn2+ depletion | Membrane-permeable Highest affinity for Zn2+, but also chelates other transition metal ions |
| EDTA EGTA |
2-5 mM 2-5 mM |
Zn2+ or Ca2+ depletion |
|
|
S. aureus α toxin Digitonin Alamethicin Saponin |
variable 10-20 μM 50 μg/mL 0.01-0.1% |
Membrane permeabilization |
A variety of permeabilization conditions have been used for Zn2+ calibrations. (3, 5, 23) |
| Pyrithione | 1-500 μM | Zn2+ ionophore | Lower concentrations may result in a more stable Rbound |