Table 1. Fold Stability and Arrhenius Plot Dynamics Data Comparisons a.
| Fold Stability | Arrhenius plot dynamics parameters | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Slope (J/mol) | 300 K | 310 K | 320 K | ||||||
| ΔGU 310K | folding | unfolding | 1/kF (μs) | 1/kU (μs) | 1/kF (μs) | 1/kU (μs) | 1/kF (μs) | 1/kU (μs) | |
| WW st29 | -10.8 | -161 | 1277 | 8.1 b | 8.9 | 230.3 | |||
| stHP1 DRGSGR-turn | -6.5 | 899 | 1574 | 63.1 | 1742 | 27.7 | 445 | 12.6 | 95.5 |
| DPATGR-turn | -8.7 | 363 | 742 | 2.4 | 69.8 | 2.0 | 40.0 | ||
| WW cp29 | -6.0 | -663 | 671 | 11 b | 21 | 213 | 49.3 | 172.7 | |
| cpHP1 DRGSGK-turn | -1.75 | 531 c | 1188 | 39.3 | 132 | 30.1 | 59.6 | 16.2 | 18.4 |
| NPATGK-turn | -5.4 | 329 | 857 | 6.4 | 76.8 | 4.5 | 36.5 | 3.7 | 17.1 |
| IpGK-turn | -7.0 | 889 | 1551 | 7.1 | 213 | 3.2 | 55.2 | 1.4 | 12.9 |
| BLB30 (n=3) | +0.62 | 372 | 865 | 112 | 162 | 92 | 72 | ||
a
The rate data at 320K provides the most complete comparison over all constructs; the rates shown are the averages over all available probes. Arrhenius inverse-log plot slopes are an average over all probes for each construct. In the case of the unfolding rates, all species display strict linearity within experimental error. There is some curvature suggested by the folding rate data for the DPATGR turn mutant of stHP1 and the NPATGK turn mutant of cpHP1.
b
Extrapolated folding rates based on the linear fit equation for ln k vs 1/T.
c
This is the clearest case of a curved ln k vs 1/T plot. The slopes at 300 and 320 K are: 80 and 450 J/mol, respectively.