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. Author manuscript; available in PMC: 2014 Jun 10.
Published in final edited form as: Chem Biol. 2009 Sep 25;16(9):1013–1020. doi: 10.1016/j.chembiol.2009.08.009

Figure 3.

Figure 3

Activities of bound His-HK and His-GPI when tethered individually. The recombinant proteins were tethered to gold chips with Ni-NTA surfaces, and washed well to remove any loosely bound or unbound proteins. Coupled enzymatic reactions were used to determine the activities of the proteins bound to the supports, with a change in absorbance at 340 nm measured spectrophotometrically, as described. Activities represent means from n = 9 samples, with 3 samples from each of 3 protein preparations. Bars indicate the standard deviations.).