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. 2014 May 21;3(5):e35. doi: 10.1038/emi.2014.35

Figure 1.

Figure 1

The human EV71 BJ110 strain infects and replicates in pig intestinal epithelial cells, PBMCs and neural cell culture in vitro. (A) Identification of virus inoculum. (a) RT-PCR detection of the EV71 BJ110 strain. The positive PCR products were purified and sequenced. The sequence shares 100% identity with the published EV71 BJ110 strain VP1 gene sequence. (b) Mock-infected Vero cell culture. (c) CPE in Vero cells 72 h post-inoculation with the EV71 BJ110 strain at a MOI of 10. (d) EV71-infected Vero cells were detected using CCIF. (B) Porcine cell cultures can be infected by the EV71 BJ110 strain. EV71 was detected in IPEC-J2 cells, PBMCs and neural cells using CCIF. (C) Growth curves for the EV71 BJ110 strain in Vero and IPEC-J2 cells suggest that EV71 infects and replicates efficiently in IPEC-J2 cells.