Table 2. Clinical signs in neonatal gnotobiotic pigs infected with the human enterovirus 71 BJ110 strain.
| Group | PPDa | Inoculation route | Dosage | Virus sheddinb | Fever (≥40 °C) | Diarrhea | Skin lesionc | Respiratory signsc | Neurological signsc | Frothy mouth |
|---|---|---|---|---|---|---|---|---|---|---|
| EV71 BJ110 | 5 | Oral-nasal | 4.5×108 FFU/oral; 5×107 FFU/nasal | 4/4 | 4/4 | 0/4 | 0/4 | 4/4 | 4/4 | 0/4 |
| EV71 BJ110 | 5 | Oral | 5×108 FFU/dose | 4/4 | 0/4 | 0/4 | 1/4 | 2/4 | 2/4 | 1/4 |
| Control | 5 | Oral | 5 mL Diluent #5d | 0/4 | 0/4 | 0/4 | 0/4 | 1/4 | 0/4 | 0/4 |
a
Postpartum day.
b
Virus shedding was detected in rectal swabs using real-time PCR.
c
See a more detailed description of these symptoms in the text.
d
Diluent #5 is the medium used to dilute the EV71 virus inoculum.