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. Author manuscript; available in PMC: 2014 Aug 20.
Published in final edited form as: Nature. 2014 Jan 5;506(7488):387–390. doi: 10.1038/nature12891

Extended Data Figure 2. Temporal analysis of meiosis in C. lusitaniae.

Extended Data Figure 2

a, C. lusitaniae a/α cells divide as stable diploid cells on YPD medium. n=3. b, On PDA medium, a/α cells are induced to enter meiosis and dyad spores (asterisks) begin to appear at 18 h. n=3. c, Time course of meiosis in wildtype, ste12Δ/ste12Δ, and STE12 complemented C. lusitaniae strains. A morphology change (polarized growth) is evident in wildtype diploid strains grown on PDA medium starting at 12 h, while spore formation is apparent at 18-36 h. In the ste12Δ/ste12Δ mutant, both sporulation and morphology change are absent. d, Global transcriptional profile of meiosis in C. lusitaniae showing induced genes (yellow) and repressed genes (blue). C. lusitaniae diploid a/α cells (RSY432) were grown on PDA (sporulating) medium and expression changes compared to those on YPD (non-sporulating) medium. Genes changing more than 4-fold in expression are shown.