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Expression of huntingtin (Htt) in HEK293T cells. The HEK293T cells were transiently transfected with 0.75 or 1.0 μg enhanced green fluorescent protein (EGFP)-Htt-exon1-20Q or EGFP-Htt-exon1–120Q and harvested 36 h later for western blot analysis. Both wild-type and mutant Htt were expressed at the expected molecular mass.
