Figure 2.

Lysosomal-associated membrane protein 2a (Lamp2a) and heat-shock cognate protein 70 (Hsc70) expression in cultured HEK293T cells transfected with huntingtin (Htt). (A and B) HEK293T cells were transfected with PRK-green fluorescent protein-exon1 Htt containing 20Q or 120Q at 0.75 or 1.0 μg. The total cell lysates were collected after 36 h of transfection for western blotting with antibodies to (A) Lamp2a and (B) Hsc70. Representative western blots are shown and the ratios of Lamp2a or Hsc70 to β-actin are noted below the blots. There was no significant difference in the Lamp2a/β-actin or the Hsc70/β-actin ratio between the cells transfected with Htt-20Q and those transfected with Htt-120Q.