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. 2014 May 15;2(4):495–499. doi: 10.3892/br.2014.276

Figure 1.

Figure 1

BA-mediated delivery of c-Met siRNA into U87-EGFRvIII cells downregulated c-Met protein expression. (A) Determination of the amount of BA, c-Met siRNA (small interfering RNA) and streptavidin used for conjugation was performed by agarose gel electrophoresis. A32, biotin-aptamer 32 (BA); B-siRNA, biotin-c-Met siRNA; B-siRNA + s = biotin-c-Met siRNA + streptavidin. A32 + S + si = biotin-A32 + streptavidin + biotin-c-Met siRNA. (B) Western blotting and (C) quantitative analysis of the changes in c-Met protein expression following BA-delivered c-Met siRNA into U87-EGFRvIII cells. Transfected and lipo, liposome-mediated group; no transfection and BA group, BA-mediated group; NCsi = liposome or BA + control siRNA + streptavidin; Bsi + S = liposome or BA + c-Met siRNA + streptavidin; Bsi = liposome or BA + c-Met siRNA; BA = BA alone. Error bars show the means ± standard deviation (n=3). *P<0.05.